Figure 4From: Follicle-stimulating hormone responsiveness in antral follicles from aryl hydrocarbon receptor knockout miceComparison of Inhba mRNA levels in response to FSH treatment in WT and AHRKO follicles. Early antral follicles from pre-pubertal WT and AHRKO ovaries were isolated and cultured in supplemented media in the presence of 0–15 IU/mL FSH for 7 days. At the end of culture, follicles were pooled per treatment group and genotype, subjected to extraction of RNA and then processed for qPCR analysis of Inhba, which is expressed as a mean relative expression ratio normalized to Actb as a loading control. Each bar represents the mean ± SEM (n=3-6 mice per genotype and 10–16 follicles at each selected treatment). The letter “a” above the bars indicates significant difference (p ≤ 0.05) from WT follicles within the same FSH treatment, and the letter “b” above the bar indicates significant difference (p ≤ 0.05) from 0 IU/mL FSH in the same genotype, using one-way ANOVA followed by Tukey’s test as a post hoc test.Back to article page