Figure 2

Sequence alignment of bovine Zona pellucida sperm-binding protein ZP domains. Top lane: Bovine ZP2 (UniProtKB: Q9BH10) ZP domain sequence. Second lane: Bovine ZP3 (UniProtKB: P48830) ZP domain sequence. Third lane: Bovine ZP4 (UniProtKB: Q9BH11) ZP domain sequence. Fourth lane: consistency. Asterisk (*) denotes identical residue; colon (:), conservative change; period (.), related substitution. Dark red indicates residues aligned in a similar fashion among all the individual MSAs (multiple sequence alignments). Dark yellow, orange and red residues can be considered to be reliably aligned. The top of the output provides the average consistency (ranging from of 0 to 100) for each sequence, indicating sequence alignment reliability (score lower than 50 is considered poor). Hereafter, amino acids are represented by single letter code for amino acid residues. The alignment was performed using the M-Coffee program [29].