Table 1 List of primers used for RT-PCR, RACE PCR and Real Time PCR
Primer no. | Gene | Primer sequences | Accession number |
|---|---|---|---|
1. | Initial 3βHSD for RT-PCR | FWD 5′-TTGATACCCCGGTTTGACCA −3′ | AY739720 |
2. | Initial 3βHSD for RT-PCR | REV 5′-TTGGCACCCCTAGATCAGTG −3′ |  |
3. | ACTB for RT-PCR | FWD 5′-ATCAAGGAGAAGCTGTGCTACGT-3′ | AB51104 |
4. | ACTB or RT-PCR | REV 5′-CGTTGCCGATGGTGATCA −3′ |  |
5. | 3βHSD RACE PCR GSP | FWD 5′-CACCAAAGCTATGATAACCTCAAT −3′ | AY739720 |
6. | 3βHSD RACE PCR GSP | REV 5′-AGGCAAGCCAGTACTCCAGAAAT −3′ |  |
7. | Universal Primer Mix (UPM) | FWD 5′-CTAATACGACTCACTATAGGGCAAGCAG | - |
|  |  | TGGTATCAACGCAGAGT-3′ |  |
8. | UPM | REV 5′-CTAATACGACTCACTATAGGGC-3′ | AY739720 |
9. | 3βHSD for RT-PCR (ORF) | FWD 5′-TTGATACCCCGGTTTGACCA −3′ |  |
10. | 3βHSD for RT-PCR (ORF) | REV 5′-TTGGCACCCCTAGATCAGTG −3′ | JF794032 |
11. | 3βHSD specific for Real Time PCR | FWD 5′-TCCCCAGTGTTTCTGATTCC −3′ |  |
12. | 3βHSD specific for Real Time PCR | REV 5′-CACCAACAAATGCACGATTC −3′ | EF522840 |
13. | Initial StAR for RT-PCR | FWD 5′-CAATGCTCCTAGCGACGTTC −3′ |  |
14. | Initial StAR for RT-PCR | REV 5′-AACAGTTGGAAGCAGCAGG −3′ |  |
15. | StAR for Real Time PCR | FWD 5′-CCCATGGAGAGGCTTTATGA-3′ | JF800676 |
16. | StAR for Real Time PCR | REV 5′-CAACTCGTGGGTGATGACTG −3′ |  |
17. | Cyclophilin for Real Time PCR | FWD 5′-CCTTCTGTAGCTCGGGTGAG −3′ | AY029366 |
18. | Cyclophilin For Real Time PCR | REV 5′-CTTGGAGGGGAGGTAAGGAG −3′ |  |