Table 1 PCR primers
Murine Gene | Primer sequence | Annealing temperature | Cycles |
|---|---|---|---|
Pgr | 5’ATGGTCCTTGGAGGTCGTAA3’ 5’CACCATCAGGCTCATCC3’ | 60 | 34 |
Esr1 | 5’CCAAAGCCTCGGGAATG3’ 5CTTTCTCGTTACTGCTGG3’ | 56 | 33 |
Esr2 | 5’GGGCCTGTTCGCCAGACTGC3’ 5’CAGGGATTTTCTTGGC3’ | 56 | 33 |
Ihh | 5’CGTGCATTGCTCTGTCAAGT3’ 5’CTCGATGACCTGGAAAGCTC3’ | 56 | 37 |
Pct1 | 5’CCTCCTTTACGGTGGACAAA 3’ 5’ GCCACATCAAGAGGTTTGGT 3’ | 56 | 40 |
Gli1 | 5’CCTGGTGGCTTTCATCAACT3’ 5’GTGGTACACAGGGCTGGAGT3’ | 56 | 38 |
β-actin | 5’GATGACGATATCGCTGCGCTG3’ 5’GTACGACCAGAGGCATACAGG3’ | 60 | 26 |