Figure 7

Stromal β-catenin deficiency results in development of luminal metaplastic lesions in Amhr2^Cre/+;Ctnnb1^d/dmutant uteri following steroid hormone treatment. Hematoxylin and eosin stained sections from Ctnnb1^flox/flox control (A, B) and Amhr2^Cre/+;Ctnnb1^d/d mutant (C, D) uteri following treatment of ovariectomized female mice with a steroid hormone regimen consistent with early pregnancy [two days of E₂ (100 ng s.c. in 100 μl sesame oil), two days no treatment, three days P₄ (1 mg s.c. in 100 μl sesame oil) and one day E₂ + P₄]. (E) Regions of bilaminar stratification (black arrows) were observed within the luminal epithelium in 66% (4/6) of the mutant uteri following E₂ (100 ng, 24 h) treatment. Representative images from n = 6-9 independent experiments.