Figure 6

Real time RT-PCR demonstrating differential mRNA expression of genes critical for embryo implantation. Control and mutant uteri were obtained from female mice treated with a steroid hormone regimen consistent with early pregnancy [two days of E₂ (100 ng s.c. in 100 μl sesame oil), two days no treatment, three days P₄ (1 mg s.c. in 100 μl sesame oil) and one day E₂ + P₄]). Shown are real time RT-PCR results for Gli1 (A), Ptch1 (B), Ihh (C) and Muc1 (D). Data represent mean + SEM of n = 4 independent experiments.