Figure 6
Effect of PD98059, Calphostin and LY294002 on the activation of primordial follicle growth. A, Ovaries were cultured for 8 days with treatments: blank control, PD98059(5 × 10-2 mmol/L), Calphostin (5 × 10-4 mmol/L)and LY294002(5 × 10-2 mmol/L). Ovarian sections were stained with hematoxylin and eosin to show the follicles at different developmental stages. Scale bar: 20 μm. HE staining X400 B, The number of follicles was counted in serial cross-sections. ** P<0.01 vs. blank control.