Effect of tocopherol supplementation during last trimester of pregnancy on mRNA abundances of interleukins and angiogenesis in ovine placenta and uterus

Table 1 Primers used for cDNA amplification of the targets by RT-PCR

Gene	Primer	Seq 5' to 3'	Product length	Accession number
IL-1b	Forward	TCACAGGAAATGAGCCGAGAA	150	NM_001009465
	Reverse	CAGCTGCAGGGTCGGTGTAT
IL-6	Forward	ACACCACCCCAAGCAGACTACT	200	NM_001009392
	Reverse	CCCAGATTGGAAGCATCCAT
IL-8	Forward	GCCAGAAGAAACCTGACAAAAAG	220	NM_001009401
	Reverse	GCAGTGTGGCCCACTCTCA
VEGF	Forward	CCTCACCAAAGCCAGCACAT	150	AF071015.1
	Reverse	CGTCTGCGGATCTTGTACAAAC
KDR	Forward	GATGCTCGCCTCCCTTTGA	250	AF233076
	Reverse	GATCCCCATGCCAGCAATC
SFlt1	Forward	GCCACGCCTGAAATCTACCA	150	AF233077
	Reverse	GGCGTTGAGCGGAATGTAGT
TNFα	Forward	GACCCTCCTCATCCCCTTCT	300	NM_001024860.1
	Reverse	AGCCCACCCATGTCAAGTTC
β-actin	Forward	CCAAGGCCAACCGTGAGA	86	NM_001009784.1
	Reverse	AGCCTGGATGGCCACGT

Ovine Interleukin (IL)-1b, IL-6, IL-8, Vascular Endothelial Growth Factor (VEGF), Kinase insert Domain Receptor (KDR), soluble Fms-Like Tyrosine kniase-1 (sFlt1), Tumor Necrosis Factor (TNF) α and β-actin cDNA were amplified by RT-PCR from the uterus and placenta total RNA using the above set of primers

ISSN: 1477-7827