Table 1 FVB/NacfBR transgenics obtained following injection with attB hFIX-containing vector with or without φC31 integrase mRNA.
From: Phage integrases for the construction and manipulation of transgenic mammals
ng/μl attB DNA/Integrase mRNA | Embryos injected | Embryos implanted | Pups harvested | hFIX positive | φC31-mediated |
|---|---|---|---|---|---|
3 DNA + 0 mRNA | 68 | 48 | 16 | 0 | 0 |
3 DNA + 3 mRNA | 144 | 102 | 39 | 3 | 1 |
3 DNA + 6 mRNA | 415 | 284 | 66 | 1 | 0 |