Figure 7
Immunohistochemical localization of SPAM1 in macaque epididymis and epididymal sperm using multiphoton confocal microscopy. In A and B (magnification seen in A) are shown the control and the test, respectively; after SPAM1 antibody staining in the corpus (Animal #2). As in Fig. 6, the images are taken in the red confocal channel but due to high autofluorescence the control is not as red as in Fig. 6. However sperm in the lumen serve as a positive control for the test whose epithelium and sperm are greener than the control. In C) are seen immature sperm from the corpus (magnification = ×1,000). SPAM1 is localized more heavily to the posterior region of the sperm head (as shown by the arrows) as was also seen for immature human sperm (data not shown).