Table 1 Primary antibodies used
Antibody | Dilution | Raised in/specifity | Company |
|---|---|---|---|
TNFR1 (p55) | 1:4 | goat anti-rat/mouse | RDI |
TNFR1 (G-20) | 1:4 | goat anti-mouse/ human | Santa Cruz |
TNFalpha | 1:4 | rabbit anti-mouse/ rat | Calbiochem |
Bcl-2 (N-19)G | 1:4 | goat anti-mouse/ human | Santa Cruz |
Bax (Δ21) | 1:8 | rabbit anti-mouse/ human | Santa Cruz |
caspase-9 p10 (H-83) | 1:7 | rabbit anti-mouse/ human | Santa Cruz |
active caspase-3 | 1:500 | rabbit anti-mouse/human | R&D Systems |
Connexin26 | 1:100 | rabbit/vertebrates | gift from O.Traub, University of Bonn |