Figure 4
Effect of treatment onexpression of integrin subunit β3 as detected by Northern blot analysis of intercaruncular endometrium. A) Three animals were used in each treatment group and received uterine infusions of either the saline control, interferon tau (IFN-τ), estrogen or ICI 182, 780. Thirty-five micrograms of total RNA from each animal were loaded per lane and biotin labeled riboprobes specific to integrin subunit β3 were used for hybridization to the membrane. A single 2.4 kb transcript was detected. B) Least squares means ± standard error for integrin subunit β3 mRNA band intensity by treatment (3 animals/treatment × 2 replicate blots) were calculated using 18S as a covariate. Asterisks indicate means that are significantly different from the Control and IFN-τ (P < 0.05).