Figure 2

Photomicrographs representing immunostaining for IL-11, IL-11Rα and cytokeratin in cycling endometrium and implantation sites of the rhesus monkey. Positive cells are shown as brown pigment with hematoxylin counterstain. (A-H) Cycling endometrium. (A and B) IL-11 staining of endometrium at ov+10: (A) luminal epithelium (le), stroma (st) and glands (g) of functionalis; (B) glands and stroma of basalis. (C and D) IL-11Rα staining at ov+10: (C) luminal epithelium, stroma and glands of functionalis; (D) stroma and glands of basalis. (E and F) IL-11 staining at ov+15: (E) luminal epithelium, stroma and glands of functionalis; (F) stroma and glands of basalis. (G and H) IL-11Rα staining at ov+15: (G) luminal epithelium and stroma of functionalis; (H) stroma and glands of basalis. Insert represents the negative control. (I-N) Implantation sites at day 24 of pregnancy. (I-K and M) IL-11 staining: (I) villi, mesenchyme (m); (J) decidua; (K) spiral arterioles (arrowed), insert represents negative control (M) gland showing punctate staining. (L and N) IL-11Rα staining: (L) spiral arteriole and perivascular cells (pv); (N) Low power view of the implantation site showing decidua (d), villi (v), and trophoblast shell (s). (O) Low power view of cytokeratin staining identifying shell and villi. Insert represents negative control. Scale bar represents 50 μm.