Figure 5
From: 4-Aminopyridine Decreases Progesterone Production by Porcine Granulosa Cells
Estradiol prevents 4-AP inhibition of progesterone production by granulosa cell cultures. Progesterone (P4) accumulation by 24 h GC cultures maintained in the basic culture media supplemented with 500 ng/mL estradiol 17β (E2), in the absence or presence of FSH (200 ng/mL), with or without the addition of 4-AP (2 mM). Data were obtained from 9 (E2+FSH) or 10 (E2, E2+4-AP, E2+FSH+4-AP) culture wells from two GC isolations. Different superscripts indicate significant difference (P < 0.05).