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Table 1 TGF-β activity in lysates of isolated testicular cells (Tissue) and in cell culture.

From: Rat testicular germ cells and sertoli cells release different types of bioactive transforming growth factor beta in vitro

 

TGF-β activity in pg × 10-6 cells/24 h

 

Tissue

Supernatant

Testicular cell type

Not activated

Heat activated

Not activated

Heat activated

Spermatocytes

ND

5.0 ± 0.6

693.0 ± 231.0

6405.0 ± 105.0

Round spermatids

ND

1.5 ± 0.2

361.4 ± 100.6

2537.5 ± 262.5

Elongating spermatids

ND

103.1 ± 9.4

596.3 ± 138.4

2981.3 ± 393.8

Sertoli cells

ND

16.7 ± 6.7

233.3 ± 66.7

1458.3 ± 208.3

  1. Supernatant, using the CCl64 mink lung epithelial cell line as a bioassay for the measurement of TGF-β activity. Spermatocytes, round spermatids, elongated spermatids and Sertoli cells were cultured for 24 h. TGF-β activity in the different preparations was measured before and after heat-activation of the samples (for details see Materials & Methods). The incubations were carried out in triplicate (values are presented as means ± SD); the data being representative of at least three different experiments. ND: not detectable.
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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