Expression of the insulin-like growth factor (IGF) system and steroidogenic enzymes in canine testis tumors

Table 3 RT-PCR analysis of canine testis tumors. PCR products were separated on agarose gels containing ethidium bromide. The gels were photographed and scanned and then relative band intensities were measured with software from Molecular Analyzer and expressed as volume counts per mm² ± the standard error of means.

Diagnosis	ARO	IGFBP1	IGFBP4	IGF-1	IGF1-R	RED 1
SCT (n = 15)	20.4 ± 8.8	10.7 ± 5.4*	17.9 ± 12.2	26.3 ± 12.1*	5.6 ± 2.2	41.3 ± 23.3*
SEM (n = 7)	17.0 ± 2.9*	8.7 ± 6.5*	17.0 ± 15.1	27.1 ± 8.0*	10.4 ± 4.1	38.3 ± 17.9*
LCT (n = 9)	19.4 ± 10.6	16.0 ± 4.9	33.3 ± 18.5*	35.4 ± 11.5	21.3 ± 3.2*	66.7 ± 20.6
MIXED (n = 5)	13.4 ± 5.3*	20.8 ± 9.4	36.8 ± 17.3*	30.8 ± 10.4	22.3 ± 8.4*	66.6 ± 6.7
NORMAL (n= 13)	27.6 ± 2.8	17.4 ± 9.4	19.5 ± 11.3	37.3 ± 9.3	7.9 ± 1.2	69.0 ± 15.9

SCT = Sertoli cell tumor, SEM = seminoma, LCT = Leydig cell tumor, mixed = a combination of testis tumors, ARO = p450-aromatase enzyme, IGFBP = insulin-like growth factor binding protein, IGF = insulin-like growth factor, IGF1-R = insulin-like growth factor receptor and RED 1 = 5α reductase type I. The number of tumors included in the measurements is indicated between brackets. Asterisk indicates significant difference between the tumor and the normal testis (p < 0.05).

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