Figure 2From: Prostaglandin F2alpha- and FAS-activating antibody-induced regression of the corpus luteum involves caspase-8 and is defective in caspase-3 deficient miceDetection of active caspase-3 in CL derived from WT and caspase-3-deficient mice following treatment with IgG, Jo2 or PGF2α. The presence of active caspase-3 was evaluated by immunohistochemistry using CM-1 antibody in sections of CL derived from WT or caspase-3-deficient mice 8 h following injection at 48 h post ovulation with IgG (A and B), Jo2 (C and D) or PGF2α (E and F). Original magnification: × 600. The insets represent a high magnification (original magnification × 1000) demonstrating the presence or absence of CM-1 positive cells (dark brown reaction). Medial sections were obtained from CL from three independent mice for analysis. Photomicrographs shown are representative of identical results in at least three separate experiments.Back to article page