Table 3 Advantages and disadvantages of density gradient centrifugation.
Advantages | Disadvantages |
|---|---|
- usually clean fraction of highly motile spermatozoa | - production of good interphases between the different media is a bit more time-consuming |
- spermatozoa from ejaculates with a very low sperm density can be separated | - a bit more expensive |
- good yield | - potential risk of endotoxins |
- leukocytes are eliminated to a large extent | - Percoll® may no longer be used IVF/ICSI |
- reactive oxygen species are significantly reduced | Â |