Fig. 7

Effects of artificial ovaries transplantation on the vagina in ovariectomised (OVX) mice. (a) Images a1-a4, b1-b4, and c1-c4 depict representative cytology images of the OVX mice, ALG mice, and CTP mice, respectively. (b) Images of the vagina stained with haematoxylin and eosin (H&E) at 6 weeks after transplantation. (c) Images of the vagina stained with H&E at 10 weeks after transplantation. (d) Proportion of mice with oestrus cycles after transplantation. (e) Vaginal indices after transplantation. (f) The thickness of epithelial cells and (g) number of epithelial layers in the vagina were calculated for each group after transplantation. The data are represented as the mean ± standard deviation. * indicates significance at P < 0.05 compared with the sham groups; # indicates significance at P < 0.05 compared with the OVX groups; $ indicates significance at P < 0.05 compared with the CTP-F groups; & indicates significance at P < 0.05 compared with ALG, determined using one-way ANOVA, followed by Student–Newman–Keuls post-hoc analysis