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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Artificial ovaries constructed from biodegradable chitin-based hydrogels with the ability to restore ovarian endocrine function and alleviate osteoporosis in ovariectomized mice

Fig. 3

Real-time polymerase chain reaction and oocyte meiotic competence. (a-i) Analysis of PCNA, FSH-R, AMH, BMP15, CYP19a1, HSD3B1, CYP17a1, CYP11a1, and STAR expression levels on day 12 of culture. (j-l) Representative images of oocyte stages after in vitro follicle maturation in CTP hydrogels. GV germinal vesicle in a mature oocyte (j) GVBD germinal vesicle breakdown (k), The first polar body (arrow) was extruded in metaphase II (M II) (l). GAPDH was used as the internal control. The data are represented as the mean ± standard deviation. * indicates significance at P < 0.05 compared with 2D, and # indicates significance at P < 0.05 compared with alginate hydrogels (ALG) determined using one-way ANOVA, followed by Student–Newman–Keuls post-hoc analysis

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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