Fig. 1

Level of miR-34c in mouse preimplantation embryos

Reverse transcription–quantitative polymerase chain reaction was used to analyze the level of miR-34c in two-cell and four-cell stage embryos and blastocysts. Data are presented as means ± standard errors of the mean (n = 3). All values were calculated against quantification cycle values. Data were normalized to endogenous RNU6B expression. *: P < 0.01 compared with other groups