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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: DNAJB7 is dispensable for male fertility in mice

Fig. 3

Dnajb7−/− mice show normal spermatogenesis. A Representative image of Dnajb7+/+ and Dnajb7−/− testes from 10-week-old mice. B Body weight of 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Dnajb7+/+, n = 5; Dnajb7−/−, n = 4. C, D Testis weight and the ratio of testis weight to body weight from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Dnajb7+/+, n = 5; Dnajb7−/−, n = 3. E Number of pups per litter from Dnajb7+/+ and Dnajb7−/− males, n = 11. F H&E staining of testes and epididymides from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Scale bar: 50 μm. G Sperm count from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Dnajb7+/+, n = 6; Dnajb7−/−, n = 4. H Sperm total motility from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Dnajb7+/+, n = 6; Dnajb7−/−, n = 5. I H&E staining shows sperm morphology from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Scale bar: 10 μm. J Immunodetection of PNA in sperm sections from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Scale bar: 10 μm. K Transmission electronic microscopic analysis of sperm in the cauda epididymis of 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Scale bar: 200 nm. L Immunodetection of γH2AX and PNA in testis sections from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Scale bar: 50 μm. M Immunostaining of the spermatogonia marker PLZF in testis sections from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Scale bar: 50 μm. N Quantification of PLZF-positive cells in seminiferous tubules from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. A total of 30 tubules per genotype were analyzed. O Immunostaining of the Sertoli cell marker SOX9 in testis sections from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. Scale bar: 50 μm. P Quantification of SOX9-positive cells in seminiferous tubules from 10-week-old Dnajb7+/+ and Dnajb7−/− mice. A total of 30 tubules per genotype were analyzed. NS, no significant difference

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