Fig. 5

Calcium oscillations are dependent on PLC pathway and CRAC channels. Ca²⁺ microfluorimetry. Representative traces of simultaneous stimulation of mEEC with trypsin (2 µg/ml) and either the PLC inhibitor U73122 (5 µM) (A), the PLC negative control compound U73343 (5 µM) (B), the IP₃R inhibitor Caffeine (C) and the CRAC inhibitor YM58483 (10 µM) (D). Ionomycin (2 µM) was added as a positive control. In E the percentage of oscillating cells within the responding cell population is shown for mEEC stimulation with trypsin (2 µg/ml), U73122 (5 µM), U73343 (5 µM), caffeine (60 mM), thapsigargin (10 µM), and YM58483 (10 µM). Experiments with thapsigargin were carried out in 0 mM extracellular Ca²⁺. Data is shown as mean ± SEM. *** p < 0.001 compared to the trypsin condition, using one-way ANOVA corrected for multiple comparisons with Holm-Sidak. For the PLC inhibitor and thapsigargin data, a two-sample t-test was performed. N = at least 3 independent experiments with a total minimum of 100 cells. Iono = ionomycin, tryp = trypsin, Thapsi = thapsigargin. F Schematic overview of PAR2 activated pathways and it’s modulator (figure created with BioRender.com)