Fig. 2
Expression of PAR2, ENaC and VDCC in mEEC. A Single cells RNA sequencing data on two different mouse endometrial cells cultures obtained from public datasets [26]. Uniform manifold approximation and projection of cell populations identified in the merged endometrial mouse datasets. B Dot plot representing the gene counts in the merged datasets for the genes F2r (Par1), F2rl1 (Par2), F2rl2 (Par3), Cacna1c (Cav1.2), Cacna1f (Cav 1.4), Scnn1a (ENaC) and Cdh1 (E-cadherin). C Expression of Par2 in Uniform manifold approximation and projection (UMAP) space of the subpopulation mouse endometrial epithelial Sox9 positive cells are shown. D, E mRNA expression of Scnn1a, Cacnca1c, Cacn1d,cacna1f and F2rl1 in isolated mEEC (D) and mESC (E). Expression is relatively quantified compared to geometric mean of the housekeeping genes Gapdh. (Data is shown as mean ± SEM. N = 3.) F In situ hybridization RNAscope images of isolated mEEC (left panels) and uterine tissue sections (right panels). Positive signals were detected for Par2 and the epithelial markers E-cadherin and Trpv6. Scale bar: 50 µm