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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Protease secretions by the invading blastocyst induce calcium oscillations in endometrial epithelial cells via the protease-activated receptor 2

Fig. 1

Trypsin induces calcium oscillations in mouse endometrial epithelial cells. A Representative traces of mEEC stimulated with trypsin (2 µg/ml) in the presence or B absence of shear stress (SS) C Percentage of oscillating cells (≥ 2 intracellular Ca2+ peaks). The presence ( +) or absence (-) of Aprotinin, shear stress and extracellular Ca2+ is shown. Data are visualized as mean ± SEM. D depicts trypsin responses in the presence (2 mM Ca2+) or absence (0 mM Ca2+) of extracellular Ca2+. In E trypsin responses were challenged with aprotinin (10 µg/ml). F Representative Ca2+ traces of mEEC upon stimulation with elastase (3U/ml). Ionomycin (2 µM) was added at the end of each experiment as a positive control. n = at least 3 independent experiments with a total minimum of 200 cells per condition. Iono = ionomycin, Tryp = trypsin, Apr = aprotinin, SS = shear stress

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