Fig. 3

Results of the ANOS1 mRNA transcripts analysis. A Sanger sequencing results of the RT-PCR products showed existence of both the wild-type (WT) transcript and the mutant transcript in the peripheral blood. The mutant ANOS1 transcript was aberrant splicing with c.1063–1086 deletion. B Schematic structures of anosmin-1 proteins. The domains of anosmin-1 protein were retrieved from the Ensembl database (Transcript ID: ENST00000262648.8). The truncated protein exhibited a mutated FnIII.2 (ΔFnIII.2) domain with amino acid deletion at 355–362