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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Insufficient HtrA2 causes meiotic defects in aging germinal vesicle oocytes

Fig. 3

HtrA2 knockdown disrupts cytoskeletal organization during oocyte meiosis. a Control and HtrA2-KD oocytes were stained with an anti-α-tubulin antibody to detect the spindle (green) and counterstained with propidium iodide to visualize the chromosomes (red). Control oocytes (n = 83) showed a typical barrel-shaped spindle. Disorganized spindles (yellow arrows) and chromosomes (white arrows) were readily observed in HtrA2-KD oocytes (n = 65). Scale bar: 25 μm. b Quantification of control and HtrA2-KD oocytes with spindle defects. c Metaphase II oocytes were labeled with phalloidin to visualize actin (green) and counterstained with propidium iodide for chromosomes (red). d Quantification of control (n = 43) and HtrA2-KD (n = 61) oocytes with normal actin cap formation. Data are expressed as the mean ± SEM of three independent experiments. *, p < 0.05. HtrA2-KD, use siRNA-1

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