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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: MiR-145 is upregulated in the retarded preimplantation embryos and modulates cholesterol levels in mice preimplantation embryos through targeting Abca1

Fig. 5

miR-145 regulates Abca1 expression to control murine embryonic development. A, B The overexpression of Abca1 was sufficient to reverse the retardation of embryonic growth observed in response to miR-145 overexpression. The images of embryos were taken at 74 h after transfer of the zygotes to KSOM. C Abca1 expression in the indicated groups was assessed via qPCR. Total number of zygotes used in (A)-(C), n = 180 for control, 121 for miR-ctr, 115 for miR145 mimic and 97 for miR145 + Abca1. Data are presented as means ± s.d. from five independent experiments. D Abca1 overexpression was sufficient to reverse miR-145 overexpression-induced increases in embryonic cholesterol levels. Total number of zygotes used in (D), n = 232 for control, 218 for miR-ctr, 209 for miR145 mimic and 195 for miR145 + Abca1. Data are presented as means ± s.d. from five independent experiments

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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