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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: MiR-145 is upregulated in the retarded preimplantation embryos and modulates cholesterol levels in mice preimplantation embryos through targeting Abca1

Fig. 3

miR-145 directly targets Abca1. A Putative sequence complementarity between miR-145 and Abca1. B miR-145 mimic transfection was adequate to suppress the luciferase activity of a reporter containing the Abca1 3′-UTR segment harboring the binding site shown in (A) (n = 3). C The overexpression of miR-145 in 293 T cells was confirmed via qPCR. D, E Abca1 mRNA and protein levels (cropped gels/blots) were assessed by means of qPCR and Western blotting after the indicated treatments. All the data are means ± s.d. from five independent experiments

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