Fig. 4

TARID promoted tube formation in trophoblasts but was not involved in proliferation and apoptosis. a Tube formation was detected in HTR8/SVneo cells after TARID overexpression and knockdown (400×). Left panel: photos of tube formation; right panel: statistics of cells. b, c Cell Counting Kit-8 was used to evaluate the cell proliferation of HTR8/SVneo (b) JAR (c) cells after TARID overexpression and knockdown. d, e Apoptosis analysis using flow cytometry on HTR8/SVneo (d) and JAR (e) cells after TARID overexpression and knockdown. Upper panels: histograms of apoptotic cells; lower panels: statistics of apoptotic cells. All experiments were conducted in triplicate; the values are shown as the mean ± SEM; vs. vector or ASO，**P < 0.01, *P < 0.05