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Fig. 6 | Reproductive Biology and Endocrinology

Fig. 6

From: Endoplasmic reticulum stress increases exosome biogenesis and packaging relevant to sperm maturation in response to oxidative stress in obese mice

Fig. 6

Palmitic acid (PA) significantly induces the ER stress in the primary EECs and promotes the EEC-derived exosome biogenesis in EECs. A Co-immunofluorescent staining of cytokeratin 8 (red) and vimentin (green) evaluated primary EECs purity. B-D PA significantly induced ER stress based on the upregulation of PERK and IRE1α in the EECs. E-F Western blot analysis detected the expression levels of the candidate proteins, PDIA3 and RRBP1, in the EECs with or without PA treatment. G-H Representative Western blot analyses of candidate protein CRELD2 and exosome marker CD63 in the EECs with or without PA treatment. I NTA determination of the concentration of the exosomes derived from the EECs. The relative quantification of the proteins was normalized to β-actin. All the data were presented as mean ± SD, *P < 0.05, **P < 0.01, ****P < 0.0001

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