Fig. 5From: Endoplasmic reticulum stress increases exosome biogenesis and packaging relevant to sperm maturation in response to oxidative stress in obese mice Association between increases in ER stress and exosomal expression levels in the caput epididymis of obese mice. A-B Representative Western blot analyses of candidate proteins PDIA3, RRBP1 in the caput epididymis of control mice and obese mice, respectively. C-D Representative Western blot analysis of candidate protein CRELD2 and exosome marker CD63 in the caput epididymis of control mice and obese mice, respectively. E-F The caput epididymis of the obese mice showed high level of ER stress with the up-regulated expression of IRE1α and PERK. G-H Representative Western blot analysis of exosome marker CD63 in the exosomes of control mice and obese mice, respectively. The relative quantification of the tissue proteins was normalized to β-actin and the exosome proteins were normalized to TSG101. All data were presented as mean ± SD, *P < 0.05Back to article page