Fig. 7

Differential apoptosis among high-fat endometrial epithelia. A Immunohistochemistry for Ki67 (top row, indicates proliferation), Cleaved Caspase 3 (CC3) (middle row, indicates apoptosis) and KRT8 (bottom row, endometrial epithelia marker) in uteri from mice on control diet (left column) or high-fat diet (middle and right columns). Two samples are shown for high-fat diet mice, one without luminal CC3 staining (middle) and one with high CC3 staining (right). Arrowheads indicate positive staining in endometrial epithelia. B Quantification of CC3 (top row) and Ki67 (bottom row) among luminal (left column), glandular (middle column) or total (right column) endometrial epithelia. Statistic is ANOVA. C Correlation between luminal CC3 and Ki67 among all mice. Statistic is Pearson’s correlation. D Correlation between luminal CC3 and endometrial F4/80 among all mice. Statistic is Pearson’s correlation. E Correlations between luminal CC3 (top row) or Ki67 (bottom row) and body mass in grams (left column) or glucose tolerance (measurement at 30 min, mg/dl) (right column) among all mice. Statistic is Pearson’s correlation. F Histograms comparing luminal CC3, luminal Ki67, endometrial F4/80, glucose tolerance (measurement at 30 min, mg/dl) and body mass (g) between high-fat diet mice with < 10% luminal CC3 or > 10% luminal CC3. Statistic is ANOVA. *p < 0.05, n.s. is non-significant