Fig. 5

Differential transcriptome analysis of obese endometrial stroma. A Principal component analysis of gene expression data from sorted endometrial stroma of control diet and high-fat diet. B Volcano plot of expressed genes. Significant differentially expressed genes (FDR < 0.05) among stroma are highlighted in red. Genes with FDR < 0.001 are denoted with gene symbols. C Enrichment analysis for GO biological processes for differentially expressed genes (n = 141). D Hierarchical clustering of 141 significant differentially expressed genes in all samples. E Broad GSEA results for high-fat diet vs. control diet epithelia (x-axis) and stroma (y-axis). Noteworthy pathways are highlighted in red. F Proportional Euler diagram of overlap between differentially expressed genes in high-fat diet vs. control diet epithelia (n = 105) and stroma (n = 141). Statistic is hypergeometric enrichment. G Heatmap of fold change values for overlapping genes from panel F