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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Obesity alters the mouse endometrial transcriptome in a cell context-dependent manner

Fig. 3

Identification of new markers for endometrial epithelia, stroma, and macrophages by RNA-seq analysis. A Principal component analysis of gene expression data from sorted endometrial epithelia, stroma, and macrophages (control diet condition) (n = 4 per cell type). B Heatmap of gene expression for known macrophage, epithelia, and stroma cell identity markers. C-E Volcano plot of differentially expressed genes between two cell types. Significantly differentially expressed genes are marked in red (FDR < 0.05). F–H Identification of signature genes for each cell type based on differentially expressed genes against other two cell types as in panels C-E. Plots are proportional Euler diagrams. Statistic is hypergeometric enrichment. I-K Enrichment analysis for Gene Ontology (GO) biological processes for signature gene sets identified as in panels F–H. L-N Left, ranking of most significantly upregulated genes with respect to epithelia vs. stroma (L), stroma vs. epithelia (M) and macrophage vs. epithelia (N), with top ten genes highlighted in red. Right, heatmap of ten most upregulated genes in given cell type and expression in all three cell types

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