Fig. 6

Differential expression of MT-dependent genes that modulate motor activities (including motor proteins) in normal vs. NOA (SCO only) human testes. A scRNA-Seq datasets from normal vs. NOA (SCO only) human testes were analyzed by Dot plot to identify differentially expressed genes encoding MT-dependent motor proteins (such as kinesins and dyneins), most notably dynein 1 (e.g., DYNC1H1, Dynein Cytoplasmic 1 Heavy Chain 1, and KIF15) as shown in Fig. 4. Dot size represents the percent (%) expressed, and color depth represents the degree of expression level from 2 to -1. The cell types were Sertoli cells (SC), peritubular myoid cells (PMC), Leydig cells (LC), endothelial cells (EC), testicular macrophages (tM), mast cells (MC), T cells (TC), smooth muscle cells (SM), spermatogonial stem cells (SSC), spermatogonia (SPG), spermatocytes (SPC), round spermatids (RS), elongated spermatids (ES) and sperm. B Heatmap of Sertoli cell genes that are known to modulate motor activities (including motor proteins), displaying differential expression in normal vs. NOA (SCO only) testes. Expression level was noted by different colors. C Violin plots of selected motor activity regulating genes expressed by Sertoli cells based on Dot plot (A) and Heatmap (B). D qPCR that compared the relative steady-state mRNA level of KIF15 in normal testes vs. NOA (SCO only). These data are consistent with the Dot plot (A), Heatmap B, Violin plot C, and IF data (Fig. 3)