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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Extracellular vesicles derived from endometrial epithelial cells deliver exogenous miR-92b-3p to affect the function of embryonic trophoblast cells via targeting TSC1 and DKK3

Fig. 2

Characterization of the EVs derived from EECs. A Western Blotting detected HSP70, TSG101 and GAPDH in the EECs-EVs fraction as well as cellular fraction derived from PTr2 cells. Calnexin (negative control) was only detected in cell lysates of PTr2 cells. B Transmission electron microscopy analysis of EECs-EVs, Scale bar = 200 nm. C Particle size of the EVs from EECs-EVs was measured by Nanoparticle tracking analysis. D EECs-EVs (20 μ g/mL) labeled with CM-Dil were added to PTr2 cells culture medium for 6 h and 12 h. scale bars = 5 μm. NC is the supernatant of the stained EVs after ultra-isolation

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