New hyaluronan-based biomatrix for 3-D follicle culture yields functionally competent oocytes

Table 3 Follicle culture and oocyte maturation in hyaluronan gel modified with laminin or ECMs

	HA	HA-LM	HA-MG	HA-PM	P-value
Follicles initially plated	70	76	76	62
Follicles retained in gel	48 (69%)	58(76%)	67 (88%)^a	58 (94%)^a	0.0006
Antrum formation	23 (48%)	17 (29%)^b	12 (18%)^c	15 (26%)^b	0.006
Ovulated complexes	31 (65%)	39 (67%)	25 (37%)^a	38 (66%)	0.001
GVBD to MI (%)	6 (17%)	12 (31%)	11 (44%)	16 (42%)	NS
Maturation to MII (%)	23 (74%)	26 (67%)	14 (56%)	22 (58%)	NS
MII oocyte diameter (µM)	91 ± 3.3	96.5 ± 4.1	89.3 ± 2.8	89.1 ± 2.8	NS

Comparison between follicles encapsulated in hyaluronan (HA) alone versus HA modified with laminin (HA-LM), mouse sarcoma ECM (Matrigel) or placental ECM (HA-PM). Percent MI and MII formation from ovulated eggs was calculated. Significantly different when compared to HA alone
^ap < 0.005
^bp < 0.05
^cp = 0.0006

ISSN: 1477-7827