Table 1 Primers used for genotyping and authentication of DKOmice
Target |  | Sequence (5′ to 3′) | Amplicon (bp) |
|---|---|---|---|
Genomic DNA | |||
 Padi2-gDNA (WT) | Forward | CTTATTCCTGGGAGCAGCAC | 233 |
Reverse | CTCTCTCTCGGCTCACCTGT | Â | |
 Padi2-gDNA (LacZ-Neo) | Forward | GATCGGCCATTGAACAAGAT | 312 |
Reverse | GTAGCCGGATCAAGCGTATG | Â | |
 Padi4-gDNA | Forward | CTCGCCTGGCACTATAAAGG | 154–160 |
Reverse | AGGATCTCTGCCCCTCTCAC | Â | |
RT-PCR | |||
 Padi1 | Forward | TGCCCACCTCTGACCAAAAG | 123 |
Reverse | GCTTGAGGCCATCAAACTGC | Â | |
 Padi2 | Forward | CAAGATCCTGTCCAATGAGAG | 189 |
Reverse | ATCATGTTCACCATGTTAGGGA | Â | |
 Padi3 | Forward | CGGAGGGTGACATCATCGAC | 88 |
Reverse | AGCATATTCACCAAGTCGGGG | Â | |
 GAPDH | Forward | TGTGTCCGTCGTGGATCTGA | 150 |
Reverse | TTGCTGTTGAAGTCGCAGGAG | Â | |
 β-actin | Forward | CATCCGTAAAGACCTCTATGCCAAC | 171 |
Reverse | ATGGAGCCACCGATCCACA | Â | |