Fig. 1From: Delayed puberty, gonadotropin abnormalities and subfertility in male Padi2/Padi4 double knockout miceDesign of Padi2/Padi4 double knockout mice. a Schematic of the CRISPR protocol using Padi2 KO zygotes. b Design and sequence of the guide RNA targeting Exon 1 of the Padi4 gene. c Sanger sequencing confirmed a four-nucleotide deletion in the DKO Padi4 Exon 1, resulting in d a frame-shift mutation and premature stop codon in the predicted amino acid sequenceBack to article page