Fig. 5

TOP2A regulates decidualization through the NF-κB signaling pathway in T-HESCs. A Volcano plot of the DEGs between the sh-TOP2A and NC T-HESCs treated with both 8-bromo-cAMP and MPA for 4 days; the DEGs were analyzed by (B) KEGG and (C) GO analysis. D Protein levels of p-p65, p65, IKK and p-IKK were examined separately by Western blotting in various T-HESCs. E Effect of TOP2A on the nuclear translocation of NF-κB p65. Decidualized cells were treated with both 8-bromo-cAMP and MPA for 4 days. The nuclear translocation of p65 was evaluated by immunofluorescence staining (p65, red fluorescence signals; DAPI, blue signals; scale bar = 200 μm). One-way ANOVA. Data are the mean ± SEM of 3 biological replicates unless stated otherwise. *p < 0.05, **p < 0.01, ***p < 0.001. ns, nonsignificant