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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: TOP2A deficit-induced abnormal decidualization leads to recurrent implantation failure via the NF-κB signaling pathway

Fig. 4

Loss of TOP2A enhances decidualization in T-HESCs. A Western blotting of TOP2A in T-HESCs. Con, untreated cells; NC, negative control cells transfected with scramble shRNA; sh-TOP2A#1–3, cells transfected with different shRNAs targeting TOP2A. B The mRNA expression of TOP2A in the NC and sh-TOP2A#3 T-HESCs was measured by RT-qPCR. C IGFBP1 protein expression in T-HESCs following transfection with NC or sh-TOP2A. The cultures either remained untreated or were decidualized for 4 days. D IGFBP1 and PRL mRNA expression in T-HESCs following transfection with NC or sh-TOP2A. The cultures either remained untreated or were decidualized for 4 days. E CytoPainter phalloidin-iFluor 488 reagent was used to label filamentous actin, and immunofluorescence was used to analyze the morphological transformation of T-HESCs. Decidualized cells were treated with both 8-bromo-cAMP and MPA for 4 days. (F-actin, green fluorescence signals; DAPI, blue signals; scale bar = 200 μm). One-way ANOVA or Mann–Whitney U test. Data are the mean ± SEM of 3 biological replicates unless stated otherwise. *p < 0.05, **p < 0.01, ***p < 0.001. ns, nonsignificant

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