Fig. 6From: Circ-CSNK1G1 promotes cell proliferation, migration, invasion and glycolysis metabolism during triple-negative breast cancer progression by modulating the miR-28-5p/LDHA pathwayMiR-28-5p suppressed the expression of LDHA. A The binding sites between miR-28-5p and LDHA 3’UTR. B and C Dual-luciferase reporter assay was performed to confirm the interaction between miR-28-5p and LDHA. D and E The expression of LDHA mRNA and protein in tumor tissues and normal tissues was measured by qPCR and western blot. F The expression of LDHA protein in MCF-10 A, MDA-MB-231 and BT-549 cells was measured by western blot. G The correlation between LDHA mRNA expression and miR-28-5p expression was analyzed by Pearson analysis. H and I The effects of miR-28-5p overexpression and deficiency on the expression of LDHA mRNA and protein were determined by qPCR and western blot. ***P < 0.001 and ****P < 0.0001Back to article page