Fig. 2

The interactions between linc00958/miR-185-5p/RSF-1 in cervical cancer cells. A-B RNAInter was applied to predict cisplatin targets and also miR-185-5p and RSF-1. C-D Starbase database provided the putative binding sites between miR-185-5p and RSF-1/linc00958. E–F Luciferase reporter gene assays. G-I&K-L RT-qPCR measured RNA levels of miR-185-5p/RSF-1 in SiHa and SiHa/DDP cells, in SiHa/DDP cells with loss or gain of linc00958. Western blot measured RSF-1 protein expression. J Spearman’s correlation analysis between linc00958 and RSF-1 in CC tissues on Gepia. M–N Impact of miR-185-5p upregulation on RSF-1. O-P CCK8 evaluated the cisplatin resistance in SiHa/DDP cells with loss of RSF-1 or gain of miR-185-5p. ****P < 0.0001, ***P =  < 0.0002, **P < 0.002, *P < 0.03