Fig. 5
Expressions of Cyp17a1 and the levels of testosterone concentrations in each group were determined and represented as histograms using immunofluorescent assays, western blot, and ELISA. A, C Nucleus labeled with DAPI appears blue and cells positive for Cyp17a1 were stained with Dylight 549 and appear as red (400X). Scale bar = 30 μm. B Survival rates of TICs were detected with the use of the CCK-8 detection kits. Survival rates of TICs (percent) expressed as a function of increasing cisplatin concentrations (mg/L). D, E Semi-quantitative analysis of Cyp17a1 as measured using western blot. F T concentrations within the control, CDDP, and CDDP + hUCMSCs groups as determined using ELISA. CDDP, cisplatin; E2, estradiol; T, testosterone; DAPI, 4', 6-diamidino-2-phenylindole; Cyp17a1, Cytochrome P450 Family 17 Subfamily A Member 1. **: P < 0.01, *: P < 0.05