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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Melatonin ameliorates diabetic hyperglycaemia-induced impairment of Leydig cell steroidogenic function through activation of SIRT1 pathway

Fig. 5

Sirt1 knockdown prevented the protective effect of melatonin on mitochondrial dysfunction caused by high glucose. A The expression levels of mitochondrial function-related proteins in TM3 cells were detected by Western blot and their gray values were analyzed. B and D Mitochondria in TM3 cells were stained by MitoTracker fluorescent probe and the percentage of cells with mitochondrial fragmentation (n = 150) was statistically analyzed. C and E Mitochondrial reactive oxygen species in TM3 cells were detected by MitoSOX, and the average fluorescence intensity was statistically analyzed. NG, normal glucose control group; HG, high glucose treatment group; HG + MT, high glucose and melatonin treatment group; siSirt1, HG + MT + siSirt1 group. A, Data are expressed as fold change relative to NG. *, P < 0.05; #, P < 0.01. Bar: B&C, 50 μm

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