Table 1 RNA sequencing analysis of miRNAs in spent blastocyst culture medium

I vs II

hsa-miR-320a

down

120.27

< 0.001

hsa-miR-320b

down

81.82

< 0.001

hsa-miR-30e-5p

down

76.80

0.008

hsa-let-7c-5p

down

75.90

0.003

hsa-miR-99b-5p

down

39.22

0.011

hsa-miR-16-5p

down

32.20

0.012

hsa-miR-509-3p

down

23.60

0.048

I vs IV

hsa-miR-320b

down

248.13

< 0.001

hsa-miR-320a

down

238.04

< 0.001

hsa-miR-203a

down

20.88

0.019

hsa-miR-184

down

4.94

0.005

hsa-miR-1910-5p

down

2.79

0.047

hsa-miR-486-5p

down

2.55

0.005

hsa-miR-320c

down

2.45

0.044

hsa-miR-148a-3p

up

90.04

< 0.001

hsa-miR-371a-5p

up

88.41

0.004

hsa-miR-192-5p

up

85.39

< 0.001

hsa-miR-28-3p

up

75.82

< 0.001

hsa-miR-378a-3p

up

74.27

< 0.001

hsa-miR-373-3p

up

65.71

< 0.001

hsa-let-7i-5p

up

52.62

< 0.001

hsa-miR-181a-5p

up

14.11

0.026

II vs IV

hsa-miR-184

down

7.62

0.001

hsa-miR-203a

down

6.81

0.017

hsa-miR-486-5p

down

3.85

0.001

hsa-miR-320b

down

3.03

0.048

hsa-miR-1910-5p

down

2.56

0.001

hsa-miR-320c

down

2.45

0.044

hsa-miR-371a-5p

up

294.07

< 0.001

hsa-miR-191-5p

up

178.42

< 0.001

hsa-miR-192-5p

up

172.80

< 0.001

hsa-miR-373-3p

up

165.56

< 0.001

hsa-miR-378a-3p

up

160.10

< 0.001

hsa-miR-16-5p

up

158.94

< 0.001

hsa-let-7c-5p

up

136.09

< 0.001

hsa-miR-372–3p

up

115.09

< 0.001

hsa-miR-148a-3p

up

114.29

< 0.001

hsa-miR-28-3p

up

114.13

< 0.001

hsa-let-7i-5p

up

103.90

0.002

hsa-miR-509-3p

up

88.80

< 0.001

hsa-miR-146b-5p

up

88.36

< 0.001

hsa-miR-30e-5p

up

65.59

0.009

hsa-miR-21-3p

up

64.15

< 0.001

hsa-let-7a-5p

up

51.99

< 0.001

hsa-let-7b-5p

up

41.52

< 0.001

hsa-miR-99b-5p

up

40.72

0.010

hsa-miR-92a-3p

up

31.57

0.038

hsa-miR-181a-5p

up

26.31

0.011