Fig. 1

Culture, differentiation and identification of BMSC in vitro. A Cell morphology of P0 and P3 generations of BMSCs (× 100, scale bar = 100 μm). B Adipogenic differentiation of BMSCs were identified by Oil red O staining in three weeks (× 200, scale bar = 50 μm). C Osteogenic differentiation of BMSCs were identified by Alizarin red staining in three weeks (× 200, scale bar = 50 μm). D Flow cytometric analysis of BMSCs. BMSCs were positive for CD29 and CD44 and negative for CD34 and CD45