Fig. 2

hCG addition complements antigen presentation potential reduction in ILC3s. Additionally to oxic conditions, in vitro-generated ILC3s were stimulated with 100 IU/mL hCG for 72 h. 18 h before flow cytometry staining cells were activated with 20 ng/mL IL-1β and IL-23. A Representative gating strategies for identification of NCR⁺ ILC3s are shown. The overlapping histograms depict the expression of extracellular markers on NCR⁺ ILC3s under 21% O₂ (stripped line) or reduced oxygen concentration (filled line). FMO controls are displayed as a filled curve. B The proportion of marker-expressing ILC3s (left) and the MFI of HLA-DR, CD80, CD40 and CD69 were analyzed (right). Effects of hCG (grey bars) were normalized to untreated cells (outlined bars) under the same oxic conditions. Bars show mean ± SEM. Data was analyzed by paired Student’s t-test. *p < 0.05, **p < 0.01. The experiment was repeated 5 (8 and 1% O₂) or 10 times (21% O₂) in duplicates