Fig. 1

Oxygen restriction reduces ILC3 antigen presentation potential. In vitro-generated ILC3s were incubated under indicated oxic conditions for 72 h. Cells were activated with 20 ng/mL IL-1β and IL-23 for 18 h prior flow cytometry staining. A Representative gating strategies for identification of NCR⁺ ILC3s are shown. The overlapping histograms depict the expression of extracellular markers on NCR⁺ ILC3s under 21% O₂ (stripped line) or reduced oxygen concentration (filled line). FMO controls are displayed as a filled curve. B Graphs show percentage of marker-expressing ILC3s (left) and the mean fluorescence intensity (MFI; right) of HLA-DR, CD80, CD40 and CD69. Data of 8 or 1% O₂ (grey) was normalized to 21% O₂ (outlined bar). Data is shown as mean ± SEM and was analyzed by paired Student’s t-test. *p < 0.05. Experiment was performed for each condition 5 times in duplicates